Glycobiology Protocols (Methods in Molecular Biology Vol 347)

A distinguished panel of international experts reviews the importance of glycobiology, an interdisciplinary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Glycobiology Protocols highlights important methodological progress that has been made in the field of glycobiology, helping scientists to answer specific questions on glycoprotein structures, on the biosynthesis of glycoconjugates, and on the functions of lipid- or protein-bound carbohydrates. Glycobiology Protocols contains specific methods for the analysis of the structures or functions of glycoconjugates, as well as of glycosyltransferases and glycosidases involved in the biosynthesis of glycans. The methods described for specific systems can be modified for investigations of similar biomolecules and tissues or cell types. Comprehensive and highly practical, Glycobiology Protocols provides researchers with a solid basis for studying the many aspects of glycobiology with state-of-the-art methods. The protocols follow the successful Methods in Molecular Biology™ series format, with each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.